解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::We have determined the size, the restriction map and the gene order of the mitochondrial genome of the yeast Saccharomyces uvarum. Sequence analysis of the mitochondrial COXII gene confirmed the position of this yeast in the Saccharomyces cerevisiae-like group, near Saccharomyces cerevisiae and Saccharomyces douglasii...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050308
更新日期:1998-01-01 00:00:00
abstract::The complete nucleotide sequence of one of three linear DNA plasmids (pRS64-2) from the plant pathogenic fungus Rhizoctonia solani was determined. The pRS64-2 DNA consisted of 2877 nucleotides. The nucleotide sequences of the middle 2.2-kb regions of the other two plasmids (pRS64-1 and pRS64-3) were also determined. C...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050299
更新日期:1997-12-01 00:00:00
abstract::The sequence of a representative mitochondrial gene COXI, encoding cytochrome c oxidase subunit I, was determined in five species that cover all the orders of the Prymnesiophyta with the exception of the Pavlovales. Through this analysis, we noticed that the 'stop' codon UGA appears frequently and, specifically, at co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050280
更新日期:1997-10-01 00:00:00
abstract::We describe a new nuclear gene, CBT1 (Cytochrome B Termination), specifically involved in the generation of mature mRNA of cytochrome b in yeast mitochondria. Disruption of CBT1 (corresponding to ORF YKL 208W) results in a respiratory deficiency (no growth on acetate and ethanol, a reduced growth on glycerol, and a mo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050262
更新日期:1997-09-01 00:00:00
abstract::Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050272
更新日期:1997-09-01 00:00:00
abstract::The DNA sequence of a 7.5-kbp region from the maize cmsT mt genome was determined. Nucleotide-sequence analysis of this region reveals the presence of a ct-like trnI (CAT) gene, orf31 and orf48 which are respectively homologous to the trnI (CAT) gene in the maize and rice chloroplast genomes, and to orf28 and orf64 in...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050256
更新日期:1997-08-01 00:00:00
abstract::Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demons...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050247
更新日期:1997-07-01 00:00:00
abstract::Caffeine is a well known base analogue and is cytotoxic to both animal and yeast cells. There are two possible mechanisms by which yeast cells tolerate caffeine concentrations higher than normal, by mutation or by physiological adaptation. We have isolated novel caffeine-resistant mutants of S. pombe which define thre...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050233
更新日期:1997-06-01 00:00:00
abstract::The Saccharomyces cerevisiae open reading frame YDL202w has been characterised in the course of the EUROFAN yeast genome analysis program. Disruption of YDL202w causes a respiratory deficient phenotype accompanied by a loss of mitochondrial DNA. This phenotype is usually found in mutants defective in mitochondrial rep...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050221
更新日期:1997-05-01 00:00:00
abstract::Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050212
更新日期:1997-04-01 00:00:00
abstract::In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent on the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promoter and introduced this chimeric construct...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050204
更新日期:1997-03-01 00:00:00
abstract::Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putative protein disulphide isomerase (PDI) from A. niger using the Saccha...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050187
更新日期:1997-02-01 00:00:00
abstract::A 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean Daphnia pulex was sequenced and found to contain the complete genes for eight tRNAs and five proteins (ATP6, ATP8, COII, COIII, ND3) and the partial sequence of COI. In combination with data described previously, approximately 50% of the D. ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050175
更新日期:1997-01-01 00:00:00
abstract::Studies from this laboratory have suggested that mitochondrial (mt) transcription in yeast (Saccharomyces cerevisiae) is governed by changing cellular cAMP levels, and that the mechanism of such transcriptional regulation requires cAMP-dependent protein kinase (PKA) activity; these observations, in turn, suggest a tra...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050161
更新日期:1996-12-01 00:00:00
abstract::The core enzyme of mitochondrial RNA polymerase in yeast is homologous to those of bacteriophages T3, T7 and SP6. In previous studies the identification of the first conditional yeast mutant for this enzyme helped to identify the corresponding specificity factor and to elucidate their interaction inside mitochondria. ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050147
更新日期:1996-11-01 00:00:00
abstract::Exonuclease II (ExoII) from Schizosaccharomyces pombe is a 5'-->3' single-stranded DNA exonuclease. We have cloned its gene, exo2, whose nucleotide sequence revealed that ExoII is a homologue of the multifunctional Saccharomyces cerevisiae Sep1 protein (also called Kem1, Xrn1, Rar5, Dst2). S. pombe exo2 null mutants w...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050134
更新日期:1996-09-01 00:00:00
abstract::Unlike most polymorphic markers in the Chlamydomonas eugametos and Chlamydomonas moewusii chloroplast DNAs (cpDNAs), the C. moewusii 6- and 21-kb extra sequences and the C. eugametos-specific CeLSU small middle dot 5 intron are transmitted to all of the few viable progeny in reciprocal crosses between the two green al...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050144
更新日期:1996-09-01 00:00:00
abstract::We have investigated chromosomal segregation during meiosis in a cross between two polymorphic haploid laboratory strains of Saccharomyces cerevisiae, FL100 and GRF18. These two strains have large chromosome-length polymorphisms for chromosomes I and III allowing for easy scoring of parental chromosomes after meiotic ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050124
更新日期:1996-08-01 00:00:00
abstract::A model for the recombination-directed replication and amplification of the mtDNA of amphimeric petite mutants of S. cerevisiae is proposed. Replication of an amphimeric master basic unit datA would be initiated in the inverted components a and A. The initiation of replication should be associated with the amphimeric ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050112
更新日期:1996-07-31 00:00:00
abstract::Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050118
更新日期:1996-07-31 00:00:00
abstract::The cellulase gene cel3 has been isolated from Agaricus bisporus and sequenced. The 5'-end of the cel3 transcript was determined by primer extension and S1 nuclease protection. Putative regulatory elements have been identified in the cel3 promoter and 3'-untranslated regions. The cel3 coding region is interrupted by s...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050100
更新日期:1996-06-01 00:00:00
abstract::We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-reme...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02426955
更新日期:1996-05-01 00:00:00
abstract::A molecular clone containing the complete sequence of a mitochondrial circular plasmid-like DNA (the R plasmid) isolated from the date-palm variety V3DP was used as a probe in Southern analyses of mitochondrial DNA prepared from other varieties. Another circular structure (the S plasmid) was detected in some of these ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02426965
更新日期:1996-05-01 00:00:00
abstract::An exceptional mode of mtDNA inheritance involving separate maternal and paternal transmission routes has been reported recently in the mussel Mytilus edulis. This mode of inheritance provides an explanation for the high levels of heteroplasmy for two highly diverged genomes observed in males of this species. Here we ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02221509
更新日期:1996-04-01 00:00:00
abstract::The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cel...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02221519
更新日期:1996-04-01 00:00:00
abstract::Mixed aeciospore isolates of Cronartium flaccidum and Peridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS1 and ITS2 were found to be highly similar between C. flaccidum and P. pini. Within samples he...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02208620
更新日期:1996-03-01 00:00:00
abstract::Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...
journal_title:Current genetics
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, A alpha and A beta. A alpha consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form h...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02221577
更新日期:1996-01-01 00:00:00
abstract::A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1-alpha (TEF1) adjacent to the 5' end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313195
更新日期:1995-12-01 00:00:00
abstract::In this paper we report the cloning, sequencing and functional characterization of CEN12 and an associated autonomously replicating sequence (ARS) from the budding yeast Saccharomyces cerevisiae. In the course of studying a dynamin-related gene, DNM1, we previously physically mapped the gene to chromosome 12. Genetic ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00518162
更新日期:1995-11-01 00:00:00
abstract::Wild-type strains of Penicillium islandicum and Penicillium frequentans, which produce anthraquinone and related compounds, were transformed to benomyl and hygromycin B resistance. Plasmids pSV50 and pBT6, with benomyl-resistant beta-tublin genes, and plasmids pAN7-1 and pDH25, with a bacterial hygromycin phosphotrans...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00518172
更新日期:1995-11-01 00:00:00
abstract::A PCR approach, employing the use of degenerate oligonucleotide mixtures, was used to isolate pph-1, a type-2A protein phosphatase (catalytic subunit)-encoding gene, from Neurospora crassa. The isolated single copy gene is 1327 nucleotides in length, contains four putative introns and encodes a 310 amino-acid polypept...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310816
更新日期:1995-10-01 00:00:00
abstract::We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal po...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326432
更新日期:1995-09-01 00:00:00
abstract::In the tribe Leptothoracini, the phylogenetic relationship of socially parasitic ants (Doronomyrmex kutteri, D. goesswaldi and Harpagoxenus sublaevis) and their host species Leptothorax acervorum has been controversial. Even more controversial is the relationship between the socially parasitic ant Chalepoxenus mueller...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309783
更新日期:1995-08-01 00:00:00
abstract::A previously unknown nuclear gene DSS-1 from Saccharomyces cerevisiae was cloned and sequenced. The gene was isolated as a multicopy suppressor of a disruption of the SUV-3 gene coding for a DEAD/H box protein involved in processing and turnover of mitochondrial transcripts. The DSS-1 gene codes for a 970 amino-acid p...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00315775
更新日期:1995-07-01 00:00:00
abstract::NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of N...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00315785
更新日期:1995-07-01 00:00:00
abstract::The Saccharomyces cerevisiae nuclear gene MRP-S9 was identified as part of the European effort in sequencing chromosome II. MRP-S9 encodes for a hydrophilic and basic protein of 278 amino acids with a molecular mass of 32 kDa. The C-terminal part (aa 153-278) of the MRP-S9 protein exhibits significant sequence similar...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311878
更新日期:1995-06-01 00:00:00
abstract::Group-I introns, containing open reading frames (ORFs) that code for homing endonucleases, are widely distributed amongst eukaryotic organellar genomes. However, endonucleases of the GIY-YIG subclass have a restricted distribution in mitochondria and bacteriophages, and have never been observed in plastids. We have fo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311888
更新日期:1995-06-01 00:00:00
abstract::By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compa...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00314446
更新日期:1995-05-01 00:00:00
abstract::The meiotic recombination gene rec10, which encodes a region-specific activator of recombination in Schizosaccharomyces pombe, has been cloned by genetic complementation and its nucleotide sequence determined. The rec10 gene was identified in a 5.6-kb cloned fragment by partial-deletion and insertion experiments. The ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311213
更新日期:1995-04-01 00:00:00